Immune Responses to Antigens of in vitro Reared Echinococcus granulosus Adult Worms in Balb/c Mice

نویسندگان

  • Bahador Sarkari Department of Parasitology and Mycology, School of Medicine | Center of Basic Researches in Infectious Diseases, Shiraz University of Medical Sciences, Shiraz, Iran
  • Hamid Reza Rahimi Department of Parasitology and Mycology, School of Medicine
  • Seyed Mahmoud Sadjjadi Department of Parasitology and Mycology, School of Medicine | Center of Basic Researches in Infectious Diseases, Shiraz University of Medical Sciences, Shiraz, Iran
  • Tahereh Mohammadzadeh Department of Parasitology and Mycology, School of Medicine
چکیده مقاله:

Background: Cystic echinococcosis (CE), also known as echinococcosis/hydatidosis, is one of the most important parasitic diseases in the world. It enhances both humoral and cellular (Th1 and Th2) responses in infected host. Different antigens of the worm may favor the Th1 or Th2 immune responses in CE patients. Objective: To evaluate the humoral and cellular immune responses of Balb/c mice against the crude and excretory/ secretory (E/S) antigens of in vitro reared Echinococcus granulosus adult worms. Methods: A total of 20 Balb/c mice divided into 5 groups of 4 mice each. Three groups of mice (n=4) were immunized with crude, E/S and an immunodominant antigen of in vitro reared Echinococcus granulosus adult worms on day 1 and 28. The fourth and the fifth groups were negative control groups and received PBS plus adjuvant, or nothing, respectively. Two weeks after the second injection, the mice were killed and their blood was collected for determining antibody responses, and their spleens were employed for proliferation assay. Total IgG were measured by indirect ELISA. Spleen cells of immunized mice were cultivated and exposed to different antigens of adult worms including E/S and crude antigens. Level of IFN-γ, IL-12, IL-4 and IL-10 were measured in the recovered cell culture supernatants by capture ELISA. Results: Total IgG assay showed the highest level of antibody produced in mice immunized with crude antigens. Proliferation assay showed a statistically significant production of cytokines in the mice immunized with crude antigens (p

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

immune responses to antigens of in vitro reared echinococcus granulosus adult worms in balb/c mice

background: cystic echinococcosis (ce), also known as echinococcosis/hydatidosis, is one of the most important parasitic diseases in the world. it enhances both humoral and cellular (th1 and th2) responses in infected host. different antigens of the worm may favor the th1 or th2 immune responses in ce patients. objective: to evaluate the humoral and cellular immune responses of balb/c mice agai...

متن کامل

Immune responses to antigens of in vitro reared Echinococcus granulosus adult worms in Balb/c mice.

BACKGROUND Cystic echinococcosis (CE), also known as echinococcosis/hydatidosis, is one of the most important parasitic diseases in the world. It enhances both humoral and cellular (Th1 and Th2) responses in infected host. Different antigens of the worm may favor the Th1 or Th2 immune responses in CE patients. OBJECTIVE To evaluate the humoral and cellular immune responses of Balb/c mice agai...

متن کامل

BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens

Background: Echinococcosis is a zoonotic parasitic disease caused by the larval stage of Echinococcus granulosus. Several native and recombinant antigens, derived from different stages of E. granulosus life cycle, have been used for vaccine trials. In vitro reared adult worms are good candidates for vaccination as they do not produce fertile egg/s and do not have any risk of contamination for r...

متن کامل

BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens.

BACKGROUND Echinococcosis is a zoonotic parasitic disease caused by the larval stage of Echinococcus granulosus. Several native and recombinant antigens, derived from different stages of E. granulosus life cycle, have been used for vaccine trials. In vitro reared adult worms are good candidates for vaccination as they do not produce fertile egg/s and do not have any risk of contamination for re...

متن کامل

evaluation of antibody against hydatid fluid, protoscolex and adult worms of echinococcus granulosus antigens by elisa in mice

echinococcosis is one of the world’s relevant zoonoses, affecting both humans and their domestic animals and caused by infection with the metacestode stage of the dog tapeworm, echinococcus granulosus. in the present study, twenty mice were randomly divided into 4 groups of 5. each mouse in groups 1, 2 and 3 was immunized with 100 μg of hydatid cyst fluid protein, protoscolex and adult worms of...

متن کامل

Protective Immunity in Mice with Whole Body of Echinococcus granulosus

Despite the establishment of extensive and successful control programmes in some countries or regions, Echinococcus granulosus still has a very wide geographical distribution. Mature E. granulosus (n = 120) obtained from Parasitology Department, School of Veterinary Medicine, Ferdowsi University of Mashhad (Iran). Soluble protein of whole body of parasite was prepared by freeze-thawing in liqui...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 8  شماره 4

صفحات  236- 243

تاریخ انتشار 2011-12-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023